Abstract
Objective Exosomes are small nanovesicles 50 to 150 nm in diameter released into the extracellular environment and are 1 of the main contributors to tumor progression and metastasis. This study seeks to assess the level of exosomes released by the tumor cells of oral squamous cell carcinoma before and after surgery and even their correlation with patient survival. Study Design Before the surgery, we took 2 plasma tubes of 1 mL each. The same procedure was repeated 7 days after the surgery. Once in the laboratory, we purified the exosomes from the frozen plasma samples and performed the determination of the exosomes (CD63 and Cav1) contained in the sample using 3 different techniques: enzyme-linked immunosorbent assay (ELISA), Western Blot, and flow cytometry. Results Kaplan-Meier curve shows that when Cav1 levels before surgery are lower than the average rate, the estimate survival is 20,344 months. In contrast, when the Cav1 is higher, these patients have an estimate survival expectation of only 1.64 months. Log rank test presents a significance of .083, close to the significance level of .05. Conclusions Although it is a pilot study, our results show that the increase in the levels of Cav1 and CD63 are related to a worse survival. Exosomes are small nanovesicles 50 to 150 nm in diameter released into the extracellular environment and are 1 of the main contributors to tumor progression and metastasis. This study seeks to assess the level of exosomes released by the tumor cells of oral squamous cell carcinoma before and after surgery and even their correlation with patient survival. Before the surgery, we took 2 plasma tubes of 1 mL each. The same procedure was repeated 7 days after the surgery. Once in the laboratory, we purified the exosomes from the frozen plasma samples and performed the determination of the exosomes (CD63 and Cav1) contained in the sample using 3 different techniques: enzyme-linked immunosorbent assay (ELISA), Western Blot, and flow cytometry. Kaplan-Meier curve shows that when Cav1 levels before surgery are lower than the average rate, the estimate survival is 20,344 months. In contrast, when the Cav1 is higher, these patients have an estimate survival expectation of only 1.64 months. Log rank test presents a significance of .083, close to the significance level of .05. Although it is a pilot study, our results show that the increase in the levels of Cav1 and CD63 are related to a worse survival.
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