Determination and detailed mechanism study of antiviral drug fosamprenavir using carbon paste electrode in the presence of Triton X-100

Abstract

The detailed electrochemical oxidation and selective determination of fosamprenavir were studied in aqueous-alcohol medium at a carbon paste electrode. Electrochemical behavior of fosamprenavir was investigated as details by different electrochemical techniques in the absence and presence of Triton X-100. The electrochemical mechanism was also carried out and aromatic amine part of the molecule was found to be involved in electrochemical oxidation of fosamprenavir. The oxidized form takes part in hydrolysis; the products are also electroactive and participate in redox reaction. Voltammetric studies were realized between pH 1.0 and 12.0. In the presence of Triton X-100, the signal increased almost 5 times. The promising analytical performance was obtained with developed method. A systematic study of the experimental parameters such as pH, scan rate, accumulation potential and accumulation time that affect the square wave stripping response was carried out and the optimized experimental conditions were arrived. Developed electroanalytical procedure is based on a carbon paste working electrode in Britton–Robinson buffer (pH∼2) using square wave voltammetry. Concentration range was linear in the range of 1×10−6–5×10−5M fosamprenavir. In sense of low detection limit (4.8×10−7M) and good repeatability (4.07%) and reproducibility (4.53%) of the results, the method was used for the determination of fosamprenavir in pharmaceutical dosage form successfully. The results are satisfying compared with that of already published high-performance liquid chromatographic results.