Abstract
RlmN, a bacterial radical SAM methylating enzyme, has the unusual ability to modify two distinct types of RNA: 23S rRNA and tRNA. In rRNA, RlmN installs a methyl group at the C2 position of A2503 of 23S rRNA, while in tRNA the modification occurs at nucleotide A37, immediately adjacent to the anticodon triplet. Intriguingly, only a subset of tRNAs that contain an adenosine at position 37 are substrates for RlmN, suggesting that the enzyme carefully probes the highly conserved tRNA fold and sequence features to identify its targets. Over the past several years, multiple studies have addressed rRNA modification by RlmN, while relatively few investigations have focused on the ability of this enzyme to modify tRNAs. In this study, we utilized in vitro transcribed tRNAs as model substrates to interrogate RNA recognition by RlmN. Using chimeras and point mutations, we probed how the structure and sequence of RNA influences methylation, identifying position 38 of tRNAs as a critical determinant of substrate recognition. We further demonstrate that, analogous to previous mechanistic studies with fragments of 23S rRNA, tRNA methylation requirements are consistent with radical SAM reactivity. Together, our findings provide detailed insight into tRNA recognition by a radical SAM methylating enzyme.
Highlights
Methylation of RNA on the four canonical bases modulates its structure and function [1], allowing it to fulfill its numerous roles in the cell
Analogous to previous mechanistic studies with fragments of 23S rRNA, tRNA methylation requirements are consistent with radical SAM reactivity
Plasmids containing Escherichia coli K-12 tRNA genes were synthesized by Gene Oracle. [14C-methyl]-SAM was purchased from Perkin Elmer
Summary
Methylation of RNA on the four canonical bases modulates its structure and function [1], allowing it to fulfill its numerous roles in the cell. These modifications are introduced by RNA methyltransferases (MTases). The exocyclic nitrogens in the nucleobases [2,3,4] as well as oxygen atoms at the 2’ position of the ribose [5,6] are nucleophilic sites. Unlike methylation of nitrogen or oxygen atoms, methylation of cytosine or uridine at the C5 position requires a different mechanism, because the target position is not nucleophilic. Methylation at the unreactive C2 and C8 positions of adenosines requires a PLOS ONE | DOI:10.1371/journal.pone.0167298 November 30, 2016
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.