Abstract
Additional effects of detergents have been examined on absorption and circular dichroism (CD) spectra of a reaction center (RC)-B890 complex of a photosynthetic bacterium, Chromatium vinosum. Anionic detergents in rather higher concentrations destroyed both B890 and RC complexes at the same time, and neutral detergents had a relatively small effect on absorption and CD spectra of the RC-B890 complexes. In contrast, zwitterionic and cationic detergents changed the absorption and CD spectra of the B890 complex at lower concentrations, when α-helical structures of the B890 polypeptides were almost conserved. In this case some amino acid side chains showed increasing molecular motions, as detected by 13C-NMR of the RC-B890 complexes from 13C-enriched cultures. NMR results from a reconstitution experiment which used a 13C-enriched B890-polypeptide and bacteriochlorophyll a with natural abundant carbons implied that histidine residues may be associated with the protein environment about the bacteriochlorophyll molecules.
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