Detergent effects on primary charge separation in wild-type and mutant Rhodobacter capsulatus reaction centers

Abstract

The primary electron-transfer processes in reaction centers (RCs) from wild-type and several mutants of Rhodobacter capsulatus have been investigated as a function of the detergent used to extract the RC protein from the membrane. Wild-type and L(M212)H mutant RCs that have been isolated using the detergent Deriphat 160-C both display somewhat slower initial charge separation (longer P* lifetimes) than the same RCs isolated using the detergent LDAO. For the F(L181)Y/Y(M208)F/L(M212)H triple mutant, the differences in the initial charge separation events for Deriphat-versus LDAO-isolated RCs are more significant. In all cases, use of Deriphat 160-C to extract the protein from the membrane yields RCs in which the QY band of P is retained at its native position near 865 nm, whereas LDAO extraction yields RCs that have the P band near 850 nm. Origins of the differences in both the ground state spectrum and the photochemistry, including possible RC–lipid associations, are considered.