Abstract
The quantification of the cellular transport capacity for the DNA staining dye Hoechst 33342 out of the cell is a widely accepted marker for recognition of stem cells. Cells with such an increased level of dye efflux (the so-called side population) can be detected on a flow cytometer equipped with a UV laser. Active transport mechanisms of this type play an important role for the elimination of anti-proliferative agents by cancer cells. A staining method for cells with high dye efflux is decribed.
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