Abstract
One of resistance mechanisms to ciprofloxacin shown by bacterium Porphyromonas gingivalis isolated from periodontitis patients is mutations of genes through changes in DNA topoisomerase. Ciprofloxacin is an effective antimicrobial for Gram-negative bacteria effectively used for clinical infections treatment. The purpose of this research was to determine the gene mutation of P. gingivalis on periodontitis patients in relation to the resistance to ciprofloxacin by using disc diffusion and, followed by RFLP-PCR on P.gingivalis samples. Based on sensitivity tests by diffusion method, it was shown that the sensitive samples (n =13) had the highest inhibition zone diameter (mean= 25 mm), while the intermediate samples (n=1) was 19 mm. Examination using RFLP-PCR for 14 samples did not reveal any mutation of gyrase A gene.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
