Abstract
Rosenmuller fossa. Epithelial malignancy is often found in Chinese populations and Southeast Asia including Indonesia. Undifferentiated nasopharyngeal carcinoma (NPC WHO-3) type is 100% associated with Epstein-Barr virus (EBV) infection. Bam-HI R Leftward Reading Frame 1 (BRLF1) lytic gene has an important function as a transition mediator of latent phase to the lytic phase in EBV cycle. Detection of BRLF1 gene by PCR can be used for NPC diagnosis. The aim of this study is to identify BRLF1 lytic genes as molecular markers of Epstein-Barr virus in nasopharyngeal carcinoma patients with conventional PCR method and to determine the sensitivity of conventional PCR method to detect BRLF1 gene. The research design was cross sectional study. A total of 22 DNA samples were isolated from venous blood of NPC patients from RSUD Prof dr Margono Soekarjo, Purwokerto with informed consent. BRLF1 gene identification is done with conventional PCR technique. The results of this research showed that BRLF1 genes as molecular markers lytic cycle of Epstein-Barr virus in nasopharyngeal carcinoma patients can be identified conventional PCR technique that will produced DNA 157 bp. BRLF1 gene was detected in 16 samples (72.73%) of 22 samples of this study.
Highlights
Karsinoma nasofaring (KNF) adalah tumor ganas yang tumbuh pada daerah nasofaring dengan predileksi di fosa Rosenmulleri
The aim of this study is to identify Bam-HI R Leftward Reading Frame 1 (BRLF1) lytic genes as molecular markers of Epstein-Barr virus in nasopharyngeal carcinoma patients with conventional PCR method and to determine the sensitivity of conventional PCR method to detect BRLF1 gene
P. Hostpathogen study : The relative expression of mRNA Bam-HI R Leftward Reading Frame 1 (BRLF1) Epstein-Barr virus : a potensial use for biomarker of tumor progresivity & polymorphisms of TCRBC andTCRGC2 penjamu genes related to genetic susceptibility on nasopharyngeal carcinoma
Summary
Penelitian ini dilaksanakan di Poli THT RSUD Prof. Margono Soekarjo Purwokerto dan Laboratorium Genetika dan Biologi Molekuler Fakultas Biologi Unsoed. Penelitian ini dilakukan selama enam bulan dari bulan September 2014Februari 2015. Rancangan Penelitian Rancangan penelitian yang digunakan adalah metode deskriptif yang berbentuk studi belah lintang (cross sectional) yaitu pengambilan sampel dilakukan satu kali tanpa perlakuan. Sampel penelitian adalah jaringan darah vena penderita KNF dengan diagnosis pasti hasil pemeriksaan Patologi Anatomi dari Poli THT RSUD Prof. Menurut Incidency Primary Cancer in Indonesia Pathology Base (1990), proporsi jumlah pasien KNF dibandingkan dengan kanker lain adalah 5,46%, maka besar sampel minimal yang diperlukan dalam penelitian ini adalah 22 orang. Variabel yang diamati dalam penelitian ini terdiri dari variabel bebas dan tergantung. Variabel bebas yang diamati adalah ukuran produk PCR (am plikon DNA) fragmen gen BRLF1, sedangkan variabel tergantung adalah eksistensi gen BRLF1 dari genom EBV
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