Abstract
Determination of enterotoxin production, diarrhoeal and emetic gene identification was studied in 41Bacillus cereusstrains isolated from raw cows’ and raw goats’ milk, pasteurized milk, dairy products during technological processing and from dairy plant equipment. Presence of enterotoxins was detected by BCET-RPLA (HBL) and ELISA immunoassay (NHE). Gene identification (nheA,nheB,nheC,hblA,hblC,hblD,bceT,cytK-1,cytK-2,entFMandces) was achieved by means of PCR. Enterotoxin HBL was detected in 32 strains, enterotoxin NHE in all 41 strains. Presence of all three genesnheA,nheBandnheCwas confirmed in 40 strains and geneshblA,hblCandhblDin 29 strains. Comparison of used methods was as follow: 1) BCET-RPLA (which detects L2 component) and PCR (positive or negative all threehblA,hblCandhblDgene detection) were identical in 30 (73%); 2) ELISA (NheA) and PCR (all threenheC,nheBandnheAgene expression) were identical in 40 (98%) cases isolated strains.
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