Abstract
Photosynthetic apparatus is susceptible to environmental stress. Light-induced delayed fluorescence (DF) in plant is an intrinsic label of the efficiency of charge separation at P680 in photosystem II (PS II). In this investigation, we have developed a biosensor that can accurately inspect acid rain pollution by means of DF in vivo. Compared with traditional methods, the proposed technique can continuously monitor environmental changes, making fast, real-time and noninvasive inspection possible. The biosensor is an all-weather measuring instrument; it has its own illumination power and utilizes intrinsic DF as the measurement marker. With soybean ( Glycine max (L.) Merr.) seedling as a testing model, which is sensitive to acid rain pollution, the relationship that delayed fluorescence properties and capability of photosynthetic apparatus after being affected by simulated acid rain with different pH value was studied. The current investigation has revealed that the changes of delayed fluorescence (equation available in paper) can probably characterize the pollution degree of simulated acid rain, Inspecting the changes in DF characteristics (φ i ) of plant leaf in vivo may be a new approach for the detection of acid rain pollution and its impact on the ecosystem.
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