Detection on clinical pathology features of central neurocytoma by using Syn, GFAP, S-100 and NSE

Abstract

Objective To investigate the clinical and pathological features of central neurocytoma (CNC),diagnosis and differential diagnosis,tissue and clinical prognosis.Methods 37 cases of brain glial cell tumors,immunohistochemistry synaptophysin (Syn),neuron-specific enolase (NSE),glial fibrillary acidic protein (GFAP),S-100 detection,detection of 9 cases of CNC.7 cases were aecompanied by differentiation characteristics of CNC.Results 6 cases were male,3 female,mean age 38.Clinical manifestations of headache,dizziness,vomiting,convulsions,coma symptoms,limb weakness.Location:2 cases occurred in the lateral ventricles,7 cases occurred in the external ventricles.The size of the tumor diameter was 3-7.5 cm.Light microscopy of tumor cells with uniform rouud,less cytoplasm,transparent,nucleus was small and round,chromatin was uniform,stippling,nuclear center,nuclear Zhou Kong halo,resembling the oligodendrocyte,tumor cells diffusely arranged densely,visible with a chrysanthemum shape,cell free zone neuropil like structure,interstitial,abundant blood vessels,slender,branched,a few appeared hyperplasia,calcifications and cystic degeneration.A few cases had nuclear atypia,mitotic and focal necrosis visible.Immunohistochemistry:Syn (strong positive 7/9,positive 2/9),S-100(strong positive 4/9,positive 4/9),NSE (weak positive 6/9),GFAP (positive 3/9,weak positive 4/9),Vimentin (strong positive 2/9,positive 1/9,weak positive 1/9),Ki67(weak positive 9/9),NF (weak positive 3/9).Follow-up of 8 cases,6 cases died,2 cases survived.Conclusions CNC morphologic features have multiple differentiation,which can occur within the ventricles,are also found in the brain of outdoor,misdiagnosed as ependymoma,oligodendroglioma and medulloblastoma tumors.Immune phenotype with differentiation,ventricle external CNC may be a glial cell differentiation gene type of the tumor,but for a prompt accompanied by glial cell differentiation of CNC,with biological behavior instability,poor clinical prognosis.Immunohistochemical Syn,GFAP are helpful to the diagnosis and differential diagnosis.High expression of GFAP and Ki67,suggests poor clinical prognosis. Key words: Brain tumor; Neurocytoma; Glial cell differentiation; Central; Immunohistochemistry; Diagnosis; Prognosis