Abstract
Advantages of testing for Zika virus (ZIKV) in urine have been reported, such as the persistence of ZIKV in this type of specimen for up to 20 days after ZIKV disease onset. We investigate 61 patients in the first 5 days post-symptom onset and find more patients testing positive for ZIKV in plasma samples (n=46), than in corresponding urine samples (n=37). For patients respectively testing positive in both plasma and urine (n=28), respective viral loads appeared similar.
Highlights
Background and discussionZika virus (ZIKV) belongs to the genus Flavivirus, a group of RNA viruses transmitted by arthropods
Specimens were investigated by real-time reverse-transcription polymerase chain reaction (RT-PCR), with a published primer set (FP: 5 ́-GAAGCCCTTGGATTCTTGAACGAGG-3 ́ and RP: 5 ́-CGACTCATCTCTTCTAGGACATATCC-3 ́) [3] and a fluorescein (FAM)/black hole quencher 1 (BHQ1)labelled Taqman probe, targeting the non-structural protein (NS)5 genetic region of ZIKV (ZIKAp 5’-FAMGGGAGAGAGAACTCAGGAGGTGG-BHQ1–3`)
A sample was considered positive for ZIKV when the mean cycle threshold (Ct) value obtained from three parallel realtime RT-PCRs was ≤ 40 cycles
Summary
Citation style for this article: Pessôa R, Patriota JV, de Souza MdL, Abd El Wahed A, Sanabani SS. Advantages of testing for Zika virus (ZIKV) in urine have been reported, such as the persistence of ZIKV in this type of specimen for up to 20 days after ZIKV disease onset. We investigate 61 patients in the first 5 days post-symptom onset and find more patients testing positive for ZIKV in plasma samples (n=46), than in corresponding urine samples (n=37). We tested for the presence of ZIKV in the urine and corresponding plasma specimens of 61 patients presenting symptoms of ZIKV disease in Brazil. The samples were collected between 1 and 5 days after symptom onset During this phase of infection, the proportion of patients testing positive in plasma samples (46/61) appeared to be higher than those testing positive in urine samples (37/61)
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