Abstract
Objectives:This study's purpose is to find out what detection of Wzm gene by PCR-based investigation in staphylococcus aureusisolates isolated from patients with eye infectionsto identify biofilm formation and thecorrelation with antibiotic resistance. Methods and Materials: 160 eye swabs specimenswere collected from iraq hospitals, including Ibn Al-Haitham Teaching Eye Hospital inBaghdad, Al-Imam Al-Sadiq Hospital andHilla Teaching Hospital in Babylon.Distributedaccording to types of ocular infections 119Conjunctivitis32Blepharitis (Eyelids Infection)7 Keratitis 2Dacryocystitis. These samples were collected between November 2021 and March 2022. S. aureus isolates were identified by inoculated on manitol salt agar, blood agar for primary screening of Staphylococcus spp. and then confirmed by biochemical tests and Vitek2 Compact System, and by specific primer for 16S rDNA gene of Staphylococcus spp.CLSI-2021were used to investigate bacterial antibiotic susceptibility testing, microtiter plates andWzm gene for biofilm production. Results:Among theculture-positive growth consist of 57(41.007%) bacterial isolates of staphylococcus aureusand all (57) isolates showedthe percentage of antibioticresistant (100%) to Cefoxitin, (96.49%)toPencillin G, (94.73%) to Oxacillin while the lowest resistant percentages were found with Nitrofurantoin (1.75%). PCR has been used to investigate Wzm gene for biofilm production instaphylococcus aureus and the results showed that 39(68.42%) has Wzm gene.
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