Abstract
This chapter discusses the detection of whole-cell metabolic rates with a silicon sensor. Using whole cells as the biologically active component of a biosensor system has a number of advantages; Eukaryotic cells contain complex signal transduction pathways that enable a cell to respond to external stimuli. A second advantage is that the information obtained is functional, not structural: a cellular response to a compound tells us not only that it is capable of binding to a receptor, but also that it triggers a particular type of response. Metabolic rates were measured by detecting the rate of change of extracellular pH when cells are confined to a small volume. A type of silicon sensor called a light-addressable potentiometric sensor (LAPS) that has a number of advantages, including compatibility with silicon micromachining was used. It is suggested that an important advantage of using a silicon sensor in this application is that silicon micro fabrication can be used.
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