Detection of virulent genetic markers like bla tem, bla imp1 of pathogenic e. coli strain isolated from Iraqi frozen chicken meat

Abstract

This research was conducted at the Department of Biology, College of Science faculty for Women, University of Kufa, Iraq. From August 2020 to April 2021, one hundred twenty samples (frozen chicken) were collected from various brands (15) and origins (5) from various locations in AL-Najaf Province. The goal of this study was to look at the incidence of Escherichia coli with extended-spectrum -lactamase genes (blaTEM, bla IMP1) in chicken meats in Iraq. The samples were obtained aseptically in a sterile plastic container then transferred to the laboratory inside an icebox for bacteriological investigation. The results revealed that Escherichia coli had the highest percentage(33, 27%)out of 120 of bacterial species isolated from frozen chicken meat then Enterobacter clocace ssp dissolvens(30, 25%) then different species as Klebsiella pneumonia ssp pneumonia( 21,18%), Klebsiella oxytoca (10,8%), Staphylococcus lantus (5,4%), Enterococcus faecalis (5,4%), Enterococcus gallinarum ( 3,3%), Enterobacter kobi (4,3%), Leclercia adecarboxylata(2,2%), Kluyvera cryocrescens (3,2.5%), Vagococcus fluvialis(2 ,2%), Enterobacter hormaechei (1,1%), Enterobacter ludwigii(1,1%). Recognition of resistance genes by PCR test yielded a negative outcome to bla IMP1 identification in all Escherichia coli isolates, while 8 (24.2 percent) of isolates were positive for blaTEM gene of Escherichia coli out of 33 examined isolates.