Abstract
Introduction: Methicillin Resistant Staphylococcus aureus (MRSA) is a well-recognized public health problem throughout the world now days. The importance and prevalence of MRSA and its antibiotic pattern were occurred frequent modication for the last two decades. In routine antibiogram assay the susceptibility were screened by Disc diffusion method but drug usage and its knowledge may differs from each physicians, leads to VRSA in MRSA, can be prevented by determining drug susceptibility by Minimal Inhibitory concentration (MIC) technique MIC and knowledge about the drug usage. Detection of Vancomycin intermediate Staphylococcus aureus (VISA) or Aim: Vancomycin Resistant Staphylococcus aureus (VRSA) in MRSA by performing MIC and Disc diffusion method. A Materials and Methods: cross-sectional study was conducted on various samples for Anti-biogram screening in the Microbiology laboratory, tertiary care hospital. To perform Antibiotic susceptibility testing (AST), Disc diffusion method in Mueller Hinton agar was done in Staphylococcus aureus isolates. MIC of Cefoxitin and Vancomycin were performed for all isolates. All the MRSA isolates were checked for susceptibility to common antimicrobial agents such as Cefoxitin (30 μg), Penicillin (10 units), Clindamycin (2 μg), Erythromycin (15 μg), Linezolid (30 μg) and Cotrimoxazole (1.25/23.75 μg). SPSS version 23 used to analyses the data. Results: Total 200 S. aureus isolated over the period of 03 months, 78 were Methicillin Resistant (39 %). A 58% were from males and 42% were from females. Cefoxitin MIC50 was 16, Vancomycin MIC50 was 0.5 and Vancomycin MIC90 was 0.25 were obtained. Conclusion: Percentage of MRSA out of S. aureus isolates was 39 %. All the MRSA isolates had a Vancomycin MIC ≤1.5 μg/mL. The current study reveals that absence of VISA or VRSA in MRSA strains.
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