Abstract
Urinary tracts infection remains a common troublesome health problem in the world. Although E.coli is normal intestinal flora but considered as the main important opportunistic active uropathogene because of it’s pathogenesity which referred to it’s different virulence factors like hemolysin, biofilm, enterotoxins, shiga like toxins, cytotoxic necrotizing factor and others. Seventy seven E.coli isolates which isolated from 125 midstream urine samples of patients suffering from different types of UTIs. The results showed that 44 isolates 57% were produced hemolysin. These isolates were differed in the efficiency of erythrocyte lysis because which depend on type of hemolysin and source of blood. Seventy two isolates 90.9% were formed biofilm with variety in thickness of biofilm layer. Each isolate produce hemolysin also formed biofilm. Presence of genes encoded for virulence traits of UPEC (hemolysin, biofilm) were examined by PCR with specific primers. The results showed that the percentage of α-hly gene encoded for α-hemolysin 50% while percentage of eae gene encoded for intimin and E-hly gene encoded for enterohemolysin were 40% and 20% respectively.
Highlights
Urinary tracts infections (UTIs) are condition where one or more structures in the urinary tracts become infected after bacteria overcome its strong natural defenses, UTIs are the most common of all infections and can occur at any time in the life of the individual [1]
Several virulence factors such as hemolysin, cytotoxic necrotizing factor, aerobactin, biofilm and different types of adhesion have been responsible for E. coli pathogenesity causing UTIs [2]
Materials & Methods Clinical isolates of E. coli A total of E. coli isolates which isolated from 125 adults patients between October 2005 to May 2006 (48 isolates from females and 29 from males).The diagnosis of UTIs was established based on clinical symptoms and laboratory investigation. the laboratory criterion for acute UTIs was defined as the presence of positive urine culture with least 105 CFU of bacteria /ml of clean voided urine, among the hospitalized patients there were 39 cystitis case, 22 cases of acute pyelonephritis and 16 cases of prostatitis
Summary
Urinary tracts infections (UTIs) are condition where one or more structures in the urinary tracts become infected after bacteria overcome its strong natural defenses, UTIs are the most common of all infections and can occur at any time in the life of the individual [1]. Hemolysin is a protein can induce osmotic lysis of erythrocyte because of its bore forming activity and cytotoxic to several types of human cell. Biofilm is a micro colony of bacteria enclosed by complex materials of protein and polysaccharide that form on living tissue and medical device such as catheter [5]. Hemolysin production was assayed by growing the E. coli isolates overnight (18 h) at 37 oC in Luria broth (LB, Himedia, India) inoculated on to blood agar plate (blood agar base, Himedia, India) containing defiberinated blood (sheep-rabbit-human) erythrocyte. Micro titer plate is other method describe by [11] for detection of hemolysin production
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