Abstract
The milk of experimentally infected rats was investigated for the presence and possible transmission of Toxoplasma gondii. Wistar (Rattus norvegicus) female rats were divided into three groups and orally inoculated with bradyzoites. Polymerase chain reaction (PCR) was employed to detect the parasite in the milk. Transmission to the offspring was verified by indirect immunofluorescence antibody test (IFAT), modified agglutination test (MAT), bioassay tests and PCR. Rat milk samples were PCR-positive, pups were serum-reactive to T. gondii and tissue samples also presented positive DNA results through PCR.
Highlights
Toxoplasmosis, a significant zoonosis, is caused by Toxoplasma gondii, whose transmission to humans can be due to consumption of infected milk [1,2,3]
Transmission to the offspring was verified by indirect immunofluorescence antibody test (IFAT), modified agglutination test (MAT), bioassay tests and Polymerase chain reaction (PCR)
Rat milk samples were PCR-positive, pups were serumreactive to T. gondii and tissue samples presented positive DNA results through PCR
Summary
Toxoplasmosis, a significant zoonosis, is caused by Toxoplasma gondii, whose transmission to humans can be due to consumption of infected milk [1,2,3]. ABSTRACT: The milk of experimentally infected rats was investigated for the presence and possible transmission of Toxoplasma gondii. Rat milk samples were PCR-positive, pups were serumreactive to T. gondii and tissue samples presented positive DNA results through PCR.
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