Abstract
Aim:The aims of the study are to detect the presence of Toxoplasma gondii antigen and to determine its distribution location in several organs of domestic cat using immunohistochemistry (IHC) method with Labeled-[Strept] Avidin-Biotin (LAB-SA).Material and Methods:Four domestic cats aged 1-2 years were used as sample in this research. The sample divided into two groups with two cats each. Cats in Group I were positive Toxoplasma based on serologically screening test, while cats in Group II were orally infected with 1×106 Toxoplasma oocyst. All samples then necropsied, and the organs including brain, liver, kidney, duodenum, jejunum, ileum, lungs, and spleen were collected for IHC method with LAB-SA.Result:The result showed that Toxoplasma antigens were detected in ileum of both serologically positive domestic cat and the experimentally infected cats. Toxoplasma was also observed in kidney of serologically positive domestic cat. In the serologically positive domestic cat, necrotic lesions were found on ileum, kidney, and liver, whereas in experimentally infected cat, the lesion was only found on ileum.Conclusion:The presence of Toxoplasma antigen is successfully detected in several organs of domestic cat using IHC method with the LAB-SA.
Highlights
The obligate intracellular parasite Toxoplasma gondii infects a broad range of mammalian and avian hosts including approximately one-third of the human population [1,2,3,4].Toxoplasmosis is a globally spread zoonosis with a clinical impact on the unborn fetuses and the immunosuppressed individuals, it is regarded as one of the leading causes of death in food-borne illness [5]
The presence of Toxoplasma antigen is successfully detected in several organs of domestic cat using IHC method with the Labeled-[Strept] Avidin-Biotin (LAB-SA)
Results of the examination of several organs by IHC staining are shown in Figure-1a and b: Macrophages are observed in some organs such as liver, lungs, and kidney of cats which serologically positive Toxoplasmosis
Summary
The obligate intracellular parasite Toxoplasma gondii infects a broad range of mammalian and avian hosts including approximately one-third of the human population [1,2,3,4].Toxoplasmosis is a globally spread zoonosis with a clinical impact on the unborn fetuses and the immunosuppressed individuals, it is regarded as one of the leading causes of death in food-borne illness [5]. The obligate intracellular parasite Toxoplasma gondii infects a broad range of mammalian and avian hosts including approximately one-third of the human population [1,2,3,4]. Toxoplasmosis is a worldwide reported zoonotic infection caused by the protozoon Toxoplasma gondii [6]. T. gondii cause a lifelong chronic infection in host by impede and suppress the immune system [7,8]. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated
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