Abstract
Abstract Cyanobacterial samples were collected from ponds, water supplies and rivers in Brittany 1991–1993. Strains belonging to Microcystis, Anabaena, Aphanizomenon and Oscillatoria were isolated and maintained in culture. Toxicity of the strains was assessed using larvae of the brine shrimp Artemia salina each and by using the bioluminescent bacterium Photobacterium phosphoreum (Cohn) Ford (Microtox® assay). It was compared with the mouse bioassay, which indicated that the toxic strains only produced hepatotoxins. Artell1ia salina larvae were sensitive to hepatotoxins, and the results showed good correlation with the mouse bioassay. In contrast, Microtox® responses were inconsistent with the mouse assay. In most cases, data obtained with Microtox® showed discrepancies: similar EC50 values were found for both toxic and non-toxic strains. Compounds other than hepatotoxins seemed to be responsible for toxic response in the Microtox® assay.
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