Abstract
The cloning efficiency of a mammalian cell culture was used as a sensitive technique for the detection of toxicants in water. The sensitivity of the technique was tested by exposing HeLa, mouse lymphoma and Buffalo green monkey cell lines to known amounts of six different toxicants. The cell culture system tested proved to be twice as sensitive as an electronic fish bioassay system. Biofilter humus tank, and activated sludge effluents as well as settled sewage showed toxic activity when tested by the cell culture system.
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