Abstract
In order to detect sugar chains specific to a tissue or a disease, we introduced the differential display of glycans using pyridylaminated (PA) sugar chains combined with two-dimensional HPLC sugar mapping. The method is fairly sensitive, and 2 mg of freeze-dried tissue was enough to detect the peaks due to PA- N-linked sugar chains, without any pretreatment of the tissue. The structure of a specific PA-sugar chain identified is determined by using two-dimensional HPLC sugar mapping combined with exoglycosidase digestion. The method was applied to find brain-type and developmentally regulated sugar chains.
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