DETECTION OF TILMICOSIN AND ENROFLOXACIN RESIDUES IN BROILER CHICKEN LIVER AND MEAT SOLED IN MARKETS AT BENHA CITY

Abou Arab N.m ,El Said A El Said ,Thabet M Gerges ,El-Katan Y.a

doi:10.21608/avmj.2017.169637

Abou Arab N.m , El Said A El Said + Show 2 more

Open Access

https://doi.org/10.21608/avmj.2017.169637

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Abstract

Fourty samples of broiler chicken liver and muscles (twenty samples for each) were collected from popular markets of Benha city, Kaluobia governorate, Egypt to detect and analyze of Tilmicosin and Enrofloxacin residues using of High Performance Liquid Chromatography (HPLC), and also to detect their influence by heat processes. Tilmicosin residues were detected in all examined liver samples with a mean concentration of 481.88 ±54.81 ppb while Enrofloxacin residues were detected in only 66.66% of liver samples with a mean concentration of 8.288 ± 1.47 ppb while all examined muscle samples were free from any detectable levels. After boiling and using of autoclave, all residues of liver samples were undetectable. This study emphasized on the importance of strict analyzing of antimicrobial residues prior to marketing and also usefulness of heat cooking with or without pressure, for complete or partial elemination of antibiotic residues.

Highlights

INTRODUCTIONExtensive use of antibiotics leads to potential health problems include allergic reactions, direct toxic effects and a change in the resistance patterns of bacteria exposed to such antibiotics (Weaver, 1992 and Fabrega et al, 2008)
Extensive use of antibiotics leads to potential health problems include allergic reactions, direct toxic effects and a change in the resistance patterns of bacteria exposed to such antibiotics (Weaver, 1992 and Fabrega et al, 2008).Many reports indicated that the resistance of microbes to antibiotics may arise from extensive adminstration of antibiotics, and this resistance may be transferred to human pathogens (Yorke and Froc, 2000)
Fourty samples of broiler chicken liver and muscles were collected from popular markets of Benha city, Kaluobia governorate, Egypt to detect and analyze of Tilmicosin and Enrofloxacin residues using of High Performance Liquid Chromatography (HPLC), and to detect their influence by heat processes

Summary

INTRODUCTION

Extensive use of antibiotics leads to potential health problems include allergic reactions, direct toxic effects and a change in the resistance patterns of bacteria exposed to such antibiotics (Weaver, 1992 and Fabrega et al, 2008). The with drawl time (WDT) is the period of time required after completion of treatment needed for tissue concentrations of the drug and/or its metabolites to deplete to less than the established MRLs. The final elimination phase depends on drug pharmaceutical formulation, dose, length of treatment, route and site of administration. Antibiotic residues in animal derived foods have been extensively recorded in many African countries; these. It is predominantly effective against Mycoplasma spp., Pasteurella haemolyticus, P. multocida and various Gram positive infections in chicken. This work was conducted to monitor Tilmicosin and Enrofloxacin residues levels in broiler chicken muscles and liver in Benha city, Kaluobia governorate, "Egypt" and study the effects of some heat treatment processes on them to ensure safety chicken for human consumption. Tilmicosin standard concentrations of 0.05, 0.1, 0.2, 0.5, 1.2 and 5 Ug/ml were preparated after said et al (2016)

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