Abstract
Thiocholine is the hydrolysis of acetylthiocholine (ATCh) by acetylcholinesterase (AChE). The detection of thiocholine can be utilized to assess the activity and inhibitors of AChE, making it a valuable recognition element for constructing biosensors used in pesticide detection[1, 2]. The enzymatic hydrolysis of ATCh is catalyzed by AChE. First, high-aspect-ratio vertically aligned silicon nanowires (VASiNWs) were successfully fabricated through metal-assisted chemical etching (MACE). To investigate the impact of nanostructures on enhancing the sensitivity of the extended gate of the field-effect transistor (EGFET), the gate terminal was connected to 3-mercaptopropyl trimethoxysilane (MPTMS) modified indium-tin-oxide (ITO)-coated VASiNWs for the electrical detection of ATCh-catalyzed thiocholine, as illustrated in Figure 1. Various solutions of ATCh-catalyzed thiocholine were collected by applying different concentrations of ATCh (ranging from 2 mM to 10 mM) to the AChE-coated wells independently for 10 minutes. Subsequently, these solutions were introduced to the MPTMS modified ITO-coated VASiNWs EGFET, and the change in drain current was measured using a semiconductor parameter analyzer (HP 4145B). The thiocholine current demonstrated a sensitivity of 29.39 uA/concentration and a linearity of 0.88. Exploring the potential of pesticides to inhibit AChE hydrolysis represents a significant breakthrough, paving the way for the advancement of biosensors in pesticide detection. References Liu, G., et al., Sensitive electrochemical detection of enzymatically generated thiocholine at carbon nanotube modified glassy carbon electrode. Electrochemistry Communications, 2005. 7(11): p. 1163-1169. Pandey, P.C., et al., Acetylthiocholine/acetylcholine and thiocholine/choline electrochemical biosensors/sensors based on an organically modified sol–gel glass enzyme reactor and graphite paste electrode. Sensors and Actuators B: Chemical, 2000. 62(2): p. 109-116. Figure 1
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