Abstract
Infiltration and proliferation of Theileria parva infected lymphocytes in bovine host lymphoid organs is one of the hallmarks of T. parva infection. The relative abundance of parasites within infected host tissues, both lymphoid and non-lymphoid is however unknown. Using quantitative PCR, we have shown that significantly higher concentrations of T. parva DNA are detected in the spleens of cattle undergoing severe disease compared to other organs.
Highlights
Infiltration and proliferation of Theileria parva infected lymphocytes in bovine host lymphoid organs is one of the hallmarks of T. parva infection
Using quantitative PCR, we have shown that significantly higher concentrations of T. parva DNA are detected in the spleens of cattle undergoing severe disease compared to other organs
In tissue samples where T. parva DNA was detected by nested PCR, T. parva DNA concentrations were estimated by quantitative real-time PCR (Odongo et al, 2009)
Summary
Once within a host lymphocyte, multi-nucleated shizont stage parasites form and induce clonal proliferation of the host cell (lymphoblasts) in which each daughter host cell receives at least one copy of parasite (Hulliger et al, 1964). Are rapidly disseminated from the site of infection to lymphoid organs throughout the body and parasites can be detected in the spleen and pre-scapular lymph nodes on the same day as sporozoite inoculation at the ear (Shatry et al, 1981). 6.6 × 102 (2.2 × 101) 6.0 × 101 (3.7) 5.3 × (2.7 × 103) 1.1 × (4.4 × 103) 1.3 × (6.7 × 102) 1.4 × (1.5 × 104) Not sampled
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