Abstract
A simple convenient synthesis is described for radioactive 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride. This reagent makes possible convenient detection of the extent of internal rearrangement and the stable addition of carbodiimide to protein when the compound is used in chemical modification studies. A survey of the reaction of carbodiimide alone with eleven typical proteins indicates that the reagent itself may be suitable for selective modification of only a few residues, including carboxylic acid side chains partially buried on the surface or in active sites. This would provide a powerful and selective tool for probing residue function in enzymes.
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