Abstract
Background: Recently, a new multicomponent RNA-containing virus was described and called as Jingmen tick virus (JMTV) supposedly belonging to flaviviruses. A virus consists of four viral particles and JMTV was firstly isolated from ticks in China and South America.
 Aims: Detection viral RNA specific for JMTV complex, sequencing genome fragments and taxonomy identification novel virus from JMTV complex in patients with Crimean-Congo hemorrhagic fever (CCHF) from southern European part of Russia.
 Materials and methods: Panel of 20 randomly selected sera from patients with confirmed Crimean-Congo hemorrhagic fever was collected in 2016 and was used for detection JMTV and CCHF viral RNA by PCR with experimental primers. Subsequent sequencing of isolated fragments of viral genomes was used for identification JMTV and CCHF virus genetic materials and phylogenetic analyses.
 Results: The viral RNAs of the CCHF virus and JMTV were detected in blood of four patients. Sequencing of the isolated PCR fragment of S segment CCHF virus allowed identifying these RNA isolates as Europe 1 lineage, subgroups Va and Vb of the CCHF virus that is a typical for the southern European part of the Russia. The nucleotide sequences of segment 2 (GP glycoprotein) of the JMTV were also detected by RP PCR and sequencing in these human sera. The new JMTV isolates were clustered together by phylogenetic analysis. The level of nucleotide identity for newly discovered JMTV isolates was only about 81-82% with comparison to the previously described European variants (Kosovo) of the JMTV.
 Conclusions: The results suggest that viral genomic RNA for new multicomponent flavivirus named as Manych virus and related to the JMTV complex was discovered in sera of CCHF patients in Russia.
Highlights
A new multicomponent RNA-containing virus was described and called as Jingmen tick virus (JMTV) supposedly belonging to flaviviruses
A virus contains of four viral particles and JMTV was firstly isolated from ticks in China
Subsequent sequencing of isolated fragments of viral genomes was used for identification JMTV and Crimean-Congo hemorrhagic fever (CCHF) virus genetic materials and phylogenetic analyses
Summary
Недавно был описан новый многокомпонентный РНК-содержащий вирус, получивший название Jingmen tick virus (JMTV), предположительно относящийся к флавивирусам. Цель исследования ― детекция генетического материала вируса JMTV, секвенирование и анализ фрагментов вирусного генома JMTV у пациентов с Крымской-Конго геморрагической лихорадкой (ККГЛ) на юге европейской части России. В сыворотках крови 4 пациентов было выявлено одновременное наличие РНК вируса ККГЛ и вируса JMTV. Нуклеотидные последовательности сегмента 2 (гликопротеин) обнаруженных изолятов JMTV-вируса при проведении филогенетического анализа кластеризовались вместе. Уровень гомологии нуклеотидной последовательности для вновь обнаруженных изолятов JMTV составлял всего около 81−82% при сравнении с ранее известными европейскими вариантами (Косово) этого вируса. Полученные результаты позволяют сделать вывод о том, что в сыворотке крови пациентов с ККГЛ обнаружены РНК вируса ККГЛ и РНК нового многокомпонентного флавивируса JMTV. Обнаружение РНК нового многокомпонентного вируса у больных Крымской-Конго геморрагической лихорадкой на юге России.
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