Detection of the presence of catalytic subunit mRNA associated with telomerase gene in exfoliated urothelial cells from patients with bladder cancer.

Abstract

Telomerase has an important role in the immortalization and oncogenesis of human cancer cells, and it appears to be a promising new marker for carcinogenesis. We investigated whether expression of the catalytic subunit of telomerase using reverse transcriptase-polymerase chain reaction (RT-PCR) can be detected in exfoliated cells in bladder washing from patients with bladder cancer. Exfoliated cells in bladder washing and voided urine samples from patients with and without bladder cancer were analyzed. To determine the number of cells required for successful detection of the subunit using RT-PCR bladder tumor cell lines were used. At least 1 x 10(4) cells were needed in the cell line study for RT-PCR of the subunit. The number of cells in bladder washing fluid and voided urine specimens was more than 5 x 10(4). Human telomerase RT (hTERT) mRNA was expressed in 62 of the 82 bladder washing fluid specimens from patients with bladder cancer but in only 2 of the 86 with benign urological disorders. Overall sensitivity for hTERT was 75.6%, that is 52.4%, 80% and 93.8% for grades 1 to 3 tumors, respectively. In contrast, human telomerase associated protein 1 mRNA was expressed in 17 of the 18 patients with and in 12 of the 23 without cancer. Overall sensitivity for human telomerase associated protein 1 was 94.4%. In 4 (57.1%) of 7 spontaneously voided urine specimens from patients with bladder cancer hTERT mRNA expression was detected. Detecting hTERT mRNA expression in exfoliated cells in bladder washing samples is more useful for the diagnosis, screening and followup of patients with bladder cancer.