Detection of the Inhibition Activity of Alcoholic Extract of Green Tea Leaves (Camellia sinensis) and Zinc Oxide Nanoparticles Against Few Bacteria and Fungi Species Isolated from Eyes Infection

Abstract

A 80 samples were collected from patients who were suffering from eyes infections in Samarra hospitals, Samarra city during the period from December 20, 2020 to January 20, 2021. All the samples were from male and female aged between 1–75 years. They were cultivated on selective and differential media in the laboratory and stained by gram stain. Some biochemical tests were done as confirmation diagnostic tests. There were 71 (88.75%) samples yielded positive growth that includes Staphylococcus aureus, Staphylococcus epidermidis and Streptococcus pneumonia 24 (30%), 16 (20%) and 9 (11.25%), respectively. The results show that 7 isolates of Haemophilus Influenzae, 6 isolates of Pseudomonas aeruginosa, 5 isolates of Escherichia coli with percentages (11.25%), (7.5%) and (6.25%) respectively. Four isolates with 5% of Candida albicans were isolated from eyes infections. The sensitivity of bacterial isolates was tested using 12 antibiotics that included quinolones, aminoglycosides, beta- lactams and tetracyclines antibiotics. Imipenem and levofloxacin were more effective than the rest of the antibiotics which range from medium to inactive. The antibacterial impact of alcoholic extracts of Camellia sinensis leaves and zinc oxide nanoparticles (ZnO NPs) on certain microbial infections were investigated. Results show that 100% concentration of alcoholic extract gives a high inhibitory effect on all isolated microbes in compares with NPs. The highest inhibition zone is S. aureus with a diameter of 35 mm and the less is 10 mm against S. pneumoniae. The alcoholic extract shows an effective result against C. albicans with a diameter of 15mm. At concentrations of 100% and 150%, zinc oxide nanoparticles inhibit S. aureus, E. coli, and C. albicans, respectively.