Abstract
BackgroundThe disease botulism is caused by intoxication with botulinum neurotoxins (BoNTs), extremely toxic proteins which cause paralysis. This neurotoxin is produced by some members of the Clostridium botulinum and closely related species, and is produced as a protein complex consisting of the neurotoxin and neurotoxin-associated proteins (NAPs). There are seven known serotypes of BoNT, A-G, and the composition of the NAPs can differ between these serotypes. It was previously published that the BoNT/G complex consisted of BoNT/G, nontoxic-nonhemagglutinin (NTNH), Hemagglutinin 70 (HA-70), and HA-17, but that HA-33, a component of the protein complex of other serotypes of BoNT, was not found.MethodsComponents of the BoNT/G complex were first separated by SDS-PAGE, and bands corresponding to components of the complex were digested and analyzed by LC-MS/MS.ResultsGel bands were identified with sequence coverages of 91 % for BoNT/G, 91 % for NTNH, 89 % for HA-70, and 88 % for HA-17. Notably, one gel band was also clearly identified as HA-33 with 93 % sequence coverage.ConclusionsThe BoNT/G complex consists of BoNT/G, NTNH, HA-70, HA-17, and HA-33. These proteins form the progenitor form of BoNT/G, similar to all other HA positive progenitor toxin complexes.
Highlights
The disease botulism is caused by intoxication with botulinum neurotoxins (BoNTs), extremely toxic proteins which cause paralysis
The BoNT/G complex consists of BoNT/G, NTNH, Hemagglutinin 70 (HA-70), HA-17, and HA-33
Separation of the BoNT/G complex by SDS-PAGE resulted in the presence of several bands (Fig. 1)
Summary
The disease botulism is caused by intoxication with botulinum neurotoxins (BoNTs), extremely toxic proteins which cause paralysis. Botulism is a disease which can be fatal if untreated and is caused by intoxication with any of the extremely toxic proteins known as botulinum neurotoxins (BoNTs). BoNTs consist of a heavy chain, which binds to receptors on the neuron, and a light chain which serves as a protease, cleaving proteins necessary for nerve signal transmission. This enzymatic cleavage leads to flaccid paralysis, which can lead to death if untreated. BoNT/C is known to cleave syntaxin [13, 14]
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