Detection of the effects of triclosan (TCS) on the metabolism of VOCs in HepG2 cells by SPI-TOFMS

Abstract

Volatile organic compounds (VOCs) emitted by organisms and cell metabolism have demonstrated great physiological and pathological values. At present, there is a great interest in the study of volatile metabolome to determine whether VOCs can serve as potential diagnostic biomarkers. In view of the sensitivity of VOCs to physiological changes, the aim of this study was to investigate alterations in VOC profiles in the in vitro headspace of HepG2 cells after exposure to triclosan (TCS). Since the in vivo biological effects of TCS are clearly defined, several TCS-related VOCs may potentially be traced back to common cellular processes. In this study, HepG2 cells were cultured in TCS-containing medium for 2 h, and the emitted VOCs in the headspace of the culture flask were detected using a single photon ionization time-of-flight mass spectrometry instrument. The control group and the TCS-treated group could be well separated by differential VOC profiles, which were related to the physiological states of the HepG2 cells. Compared to the control group, eleven and ten specific VOCs were identified in the 20 μm and 50 μm TCS-treated groups, respectively. Among them, five specific VOCs (m/z 62, 64, 70, 121 and 146) were commonly observed in these two TCS-treated groups. These results indicate that TCS can cause changes in cellular metabolic VOCs, and different concentrations of TCS lead to different VOCs profiles. Based on the findings of the study, the detection of VOCs in cell metabolism can be used as an auxiliary tool to explore the mechanism of drug action, and also as an exploratory method to determine whether drugs play a role in disease treatment.