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Abstract
The laser-induced fluorescence (LIF) was tested as a means to detect the effects of a pink-pigmented facultative methylotrophic bacteria ( Methylobacterium) in blue agave plants, and compared with conventional techniques of leaf tissue analysis. A He–Ne laser emitting at 632.8 nm was used as the excitation source. In vivo fluorescence emission spectra were recorded in the 660–790 nm range. Fluorescence maxima were at 690 and 740 nm. The inoculated plants showed a significant change in fluorescence as compared to that of non-inoculated plants. The ratios of fluorescence intensities F 690/ F 740 were calculated for all treatments. A comparison between conventional techniques and this non-invasive method is presented. The results of this work showed that the beneficial effects of PPFM bacteria on agave plants are detectable by LIF.
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