Detection of the bladder volume from the neural afferent activities in dogs: experimental results

Abstract

Objective: We evaluate the bladder volume and pressure through recording the bladder afferent activity in the sacral nerve roots in acute experiments of paraplegic dogs. These measurements are intended to report the status of the bladder and to adjust the stimulation parameters of an implantable electric stimulator.Methods: The extraction of neural information for feedback in functional electrical stimulation is limited by the poor signal to noise ratio (SNR) in the sacral nerve recordings. We propose to inject a very low amplitude sinusoidal current with high SNR to the bladder through the nerve using a tripolar cuff electrode wrapped around the S2 nerve root. The application of this current (0.4 μA peak to peak, 30 Hz) allows detecting bladder afferent activity in its amplitude and the tissues impedance of the nerve. Acute experiments in dogs were performed to evaluate the proposed method. In each dog, the bladder infusion with saline was carried out at both slow and high filling rates. At the same time, the changes in the amplitude of the sinusoidal output voltage VOUT were recorded through the cuff nerve electrode.Results: The data obtained from 26 acute experiments using eight dogs demonstrate that the amplitude of the recorded sinusoidal voltage VOUT varies proportionally with the bladder pressure during the bladder filling with saline solution. It also demonstrates that the bladder volume can be estimated from the increasing amplitude of the recorded VOUT.Conclusion: This study shows that the increase in the VOUT is proportionally related to the increase in bladder pressure. The difference between the recorded VOUT during the bladder filling and the baseline VOUT can be a useful indicator of the changes in the bladder volume.