Abstract
A sensitive high-pressure liquid chromatographic assay for the detection and quantitation of the amine derivative of misonidazole in human urine is reported. The amine was converted to its dansyl derivative and was separated on a reverse-phase μBondapak phenyl column. Absorbance at 365 nm was monitored for the detection of the dansyl derivative. Using this procedure, the amine derivative has been identified as a urinary metabolite of misonidazole.
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