Abstract
The reverse transcription-polymerase chain reaction (RT-PCR) was adapted for detection of sugarcane mosaic potyvirus (SCMV) and Fiji disease fijivirus (FDV) in total nucleic acid extracts from diseased sugarcane. The denaturation method developed for RT-PCR of FDV-specific double-stranded RNA (dsRNA) was suitable for the amplification of SCMV RNA and also amplified SCMV-specific replicative form (double-stranded) RNA. RT-PCR amplification of total nucleic acid extracts from SCMV-infected sugarcane yielded a 359-bp product and detected the presence of the virus in a 1:10 4 dilution of a solution containing the total nucleic acids extracted from 250 mg of SCMV-infected tissue [...]
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