Abstract
Avian leukosis virus subgroup J (ALV-J) is an exogenous avian leukosis virus that causes significant economic losses. In this study, a whole blood polymerase chain reaction (PCR) assay without DNA extraction was developed, optimized, and was compared with conventional methods for the detection of ALV-J infection. The evaluation assay indicated that the whole blood PCR had similar sensitivity and specificity when compared with virus isolation and conventional PCR. Taken together, the whole blood PCR method was very specific, easy to perform, rapid and of low cost, which will be useful for routine diagnosis of ALV-J, especially for large scale sampling. Key words: Av/ian leukosis virus subgroup J, polymerase chain reaction, whole blood.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
