Abstract
Cell imagery is generally based on the use of molecular fluorescent probes. These probes bind to the external membrane of the cell and their optical properties vary with one of the electro-chemical properties of the cell—such as calcium concentration or electrical membrane potential. In using a voltage-sensitive probe, one can image the spatial changes in the electrical potential on the membrane of an excited neuron. Because of the low transduction power of such probes, the changes in potential induce very small changes in intensity. The purpose of the chapter is to detect such changes between two charge coupled device (CCD) images corrupted by a signal-dependent noise and with nonuniform illumination. This method is a complete image processing tool that gives significant results, but it is unfortunately limited by its computational efficiency, both in time and computer storage space. However, this algorithm conserves the photometrics and provides a calibration in size and intensity of active sites by extraction of significant structures.
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Schedule a callMore From: Proceedings IWISP '96, 4 - 7 November 1996; Manchester, UK
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