Detection of Salmonella in Refrigerated Preenrichment and Enrichment Broth Cultures

Abstract

Refrigeration (4 C) of non-selective and selective enrichment broth cultures for 72 h did not markedly affect detection of Salmonella in 160 contaminated high and low moisture foods. Detection in refrigerated preenrichment (non-selective) broth cultures of poultry and high and low moisture foods was 90, 95 and 100%, respectively; homologous results for refrigerated selective enrichment broth cultures were 90, 100 and 100%. All but one of the 22 negative results were obtained with poultry and two of the six laboratories participating in poultry analysis contributed 19 of the 21 negative results. Refrigeration of broth cultures provides greater operational flexibility by increasing the number of days on which analyses can be initiated without engendering work outside a normal work week.