Abstract
A total of 150 random samples were collected from different supermarkets and retailers of differentsanitation levels at Gharbeia governorate, Egypt as follows 70 samples of minced meat, 40 samples ofsausage and 40 samples of beef burger. The aforementioned samples were subjected to bacteriologicaland serological applications to assess the prevalence of S. Enteritidis. The obtained results revealed thatthe incidence of S. Enteritidis in the examined samples of minced meat, sausage and beef burger were1/70 (1.4%), 1/40(2.5%) and 0/40(0%) respectively. The isolates were submitted for serological analysisand revealed that Salmonella Enteritidis O 1,9,12 ad monophasic H:g, m. The antibiogram sensitivitytest was applied upon the two isolates of S. Enteritidis and revealed that they are sensitive tochloramphinicol, amoxicillin, levofloxacin, ciprofloxacin, enrofloxacin and gentamycin but they wereresistant to oxytetracycline. The virulence genes of S. Enteritidis isolates were determined by usingmultiple PCR technique for the two serologically detected Salmonella Enteritidis by using the followinggenes inv A, sef A, sop B and bcf C at 284bp, 310bp, 517bp, 467bp, respectively.
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