Abstract
A novel method was developed for the detection of Salmonella bacteria using gold-coated magnetic nanoparticle clusters (Au/MNCs) and lateral flow filters.
Highlights
Lateral ow immunoassay (LFA), known as immunochromatographic assay, is the most common commercially available point-of-care (POC) diagnostic tool because it is low-cost, disposable, and requires no specialized equipment, and it is used in various applications, such as in the detection of proteins,[1,2] antibiotics,[3] metal ions,[4,5] and toxins.[6,7,8,9] A typical lateral flow immunoassay (LFA) uses speci c biological components such as antibodies and DNAs to observe a signal at test and control lines on a nitrocellulose (NC) membrane
The free antibody fragment-functionalized gold-coated magnetic nanoparticle clusters (Au/MNCs) rose to the pressed test line, while the Salmonella–Au/MNC complexes remained in the solution because they were too large to pass through the pores inside the lateral flow filter
The flow of free Au/MNCs was blocked at the test line because of the reduced pore size, and their accumulation caused the test line to darken
Summary
Lateral ow immunoassay (LFA), known as immunochromatographic assay, is the most common commercially available point-of-care (POC) diagnostic tool because it is low-cost, disposable, and requires no specialized equipment, and it is used in various applications, such as in the detection of proteins,[1,2] antibiotics,[3] metal ions,[4,5] and toxins.[6,7,8,9] A typical LFA uses speci c biological components such as antibodies and DNAs to observe a signal at test and control lines on a nitrocellulose (NC) membrane. The working principle of LFA is simple, it cannot be applied in a straightforward manner for the detection of foodborne bacteria in real samples because the concentration of bacteria rinsed off of food samples is very low compared to the concentration of food debris. This problem may be circumvented by using magnetic nanoparticles (MNPs), which allow. We used half-antibody fragment-functionalized gold-coated magnetic nanoparticle clusters (Au/MNCs) to capture Salmonella bacteria in milk and determined the concentration using a lateral ow lter with a reduced pore size at the test line. The color intensity at the test line was inversely proportional to the Salmonella concentration, and the limit of detection for Salmonella in milk was determined to be 103 CFU mLÀ1 by the naked eye
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