Detection of rs1225537 SNP in the Hispanic Population of Southern California with Type II Diabetes

Abstract

In Hispanics, type II diabetes is the fifth leading cause of death among Hispanics in the United States due to the genetic influence. Through this, scientists study the DNA to locate and study the specific DNA sequences called single nucleotide polymorphisms, SNP, associated with type II diabetes. The single nucleotide polymorphism, rs12255372, in recent studies has been associated with type II diabetes, breast cancer, and prostate cancer. The allele involve in the risk of Diabetes is G and T. DNA was extracted from the collected buccal samples. The 47 samples were run through a polymerase chain reaction (PCR) and confirmed by gel electrophoresis. After the amplification, the products were digested with the restriction enzyme MluCl. The G/G allele was the most predominant allele in both groups. Increasing sample size will allow for the data to be more concrete.