Abstract
Advancements in next-generation sequencing and bioinformatics have expanded our knowledge of the diversity of viruses (pathogens and non-pathogens) harbored by mosquitoes. Hubei reo-like virus 7 (HRLV 7) was recently detected by the virome analysis of fecal samples from migratory birds in Australia. We now report the detection of RNA-dependent RNA polymerase sequences of HRLV 7 in pools of Aedes aegypti and Culex quinquefasciatus mosquitoes species from the Brazilian Amazon forest. Phylogenetic inferences indicated that all HRLV 7 strains fall within the same independent clade. In addition, HRLV 7 shared a close ancestral lineage with the Dinovernavirus genus of the Reoviridae family. Our findings indicate that HRLV 7 is present in two species of mosquitoes.
Highlights
Mosquitoes are well recognized arthropod vectors of viral pathogens worldwide [1,2]
We processed a total of 191 pools of mosquitoes from Macapá and Santos that were submitted for Next-Generation Sequencing (NGS)
In six pools we detected sequences (3372 nucleotide length on average) that were nearly identical to the RdRp segment of the mosHB235771 Hubei reo-like virus 7 strain (KX884635)
Summary
Mosquitoes are well recognized arthropod vectors of viral pathogens worldwide [1,2]. In addition, these insects are widely known to harbor a large diversity of seemingly non-pathogenic viruses [3,4,5].In Brazil, climatic Viruses147 geographic conditions are very favorable for the proliferation and dissemination of mosquitoes and their viral microbiota: frequent rainfall, year-round high temperature and denseMosquitoes well recognized arthropod vectors of viralcaused pathogens worldwide [1,2]. Mosquitoes are well recognized arthropod vectors of viral pathogens worldwide [1,2] These insects are widely known to harbor a large diversity of seemingly non-pathogenic viruses [3,4,5]. Brazil has faced multiple epidemics by mosquito-borne viruses addition, these insects are widely known to harbor a large diversity of seemingly non-pathogenic such as dengue virus, Zika virus and yellow fever virus [6,7,8,9]. These factors highlight the need for viruses [3,4,5]. Metagenomic analysis allows for and dissemination of mosquitoes and their viral microbiota: frequent rainfall, year-round high the detection and genetic characterization offew novel viruses using high-throughput
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
