Detection of rice seed vigor by low-field nuclear magnetic resonance

Ping Song,Hongwei Yang,Jing Xu,Tao Yang,Kaitian Wang,Peng Song

doi:10.25165/j.ijabe.20181106.4323

Abstract

A new method to predict the seed vigor of rice was developed to control adulteration during the seed trading process and to address the deficiencies of traditional manual detection methods. Low-field nuclear magnetic resonance (LF-NMR) technique was used to detect the vigor of rice seeds. Four varieties (Beijing-1, Qianchonglang-2, Yanfeng-47 and Shennong-265) of rice seeds from the Rice Research Institute of Shenyang Agricultural University were chosen for the experiment. The transverse relaxation time T2, T21 and T22 were observed in the experiment. The peak start time of free water (transverse relaxation time T22), signal amplitude of bound water (transverse relaxation time T21), and moisture content decreased with the decrease in the vigor of the seeds. There were no obvious trends observed for the top of the peak and the end point of the transverse relaxation time T22. In addition, the start, top, and end time of the peak (transverse relaxation time T21), and the signal amplitude of bound water showed no consistent changes. The results indicated that LF-NMR could be used as a method to distinguish the vigor of rice seeds rapidly. This study provided theoretical basis and technical support for the rapid detection of rice seed vigor. Keywords: nondestructive detection, nuclear magnetic resonance, transverse relaxation time, signal amplitude, rice, seeds vigor DOI: 10.25165/j.ijabe.20181106.4323 Citation: Song P, Song P, Yang H W, Yang T, Xu J, Wang K T. Detection of rice seed vigor by low-field nuclear magnetic resonance. Int J Agric & Biol Eng, 2018; 11(6): 195–200. (6): 195–200.

Highlights

Seed vigor is an indicator of the integrative performance of the seed during the period of germination and emergence of the seedling
It was found that the transverse relaxation time of the NMR inversion spectrum and the signal amplitude were different
This shows that the low-field nuclear magnetic resonance (LF-NMR) technology can be used to quickly distinguish the adulteration of rice seeds

Summary

Introduction

Seed vigor is an indicator of the integrative performance of the seed during the period of germination and emergence of the seedling. The ISTA handbook (ISTA procedure, third edition, 1995) for the determination of seed vigor recommends seven kinds of seed vigor testing methods, which use conductivity and accelerated aging as the measurement techniques. These detection methods have the advantage of being intuitive, but they have the disadvantages of manual operation, long operation time, subjective factors, extensive workload, and low efficiency[7]. This research uses LF-NMR technology to overcome the disadvantages of the above studies, which are low efficiency and dependence on artificial detection techniques such as electrical conductivity measurement and accelerated aging test.

Methods

Results

Conclusion