Detection of resistant strains of tubercle bacilli by gene mutation hybridization

Abstract

Introduction Tuberculosis is the leading cause of death in the world from asingle infectious agent. Aim of the work Use molecular technique to provide information to detect the recent transmission events and to describe part of population structure of tubercle bacilli. Material and methods DNA fingerprinting of mycobacterium tuberculosis with the transmission IS6110 provide tool for identification of transmission and investigation of tuberculosis outbreaks. Seventy DNA Samples of patients and three samples of the relatives were subjected to pcr amplification followed by restriction to genomic DNA by PVULL enzyeme. Hybridization was done by using IS611 probe. The copy number was detected by radiation PCR amplification of the particular target region its corresponding gene (rPOB, Katg, rpst) was followed by automated DNA sequencing of the amplification to detect any mutation. Strains contains 8–11 IS6110.