Detection of Protoparvovirus in wastewater and human adenovirus in a green leafy vegetable in an Environmental Education Center in southern Brazil

Abstract

Abstract The presence of coliforms, Mastadenovirus (AdV), and Canine protoparvovirus (CPV) in an Environmental Education Center, which contains city kennel, was investigated. Surface water and wastewater, dogs’ stool, and lettuce samples were collected from the irrigation system in both periods October 2017 and February 2018. Water samples were analyzed for the presence of human AdV (HAdV) and canine AdV (CAV), CPV, total coliforms (TC), and Escherichia coli; lettuce for AdV; and dog stool for CAV and CPV. Sixty-seven samples were analyzed: dog stool (n = 33); water (n = 10), and root, stem, and leaf (n = 24) originated from eight lettuce samples. Coliforms and E. coli were tested by Colilert and viruses by PCR assays, respectively; and the amplicon was sequenced. E. coli, TC, and viruses were detected in both collections. Viruses were detected in 34.3% of samples, of which HAdV-C was present in 13% of water samples; HAdV-E in 8.7% (water and lettuce); CAV-1 in 13% (dog stool and water); CPV-2a in 56.5% (dog stool). Raccoon CPV-like and AdV not characterized were detected in one wastewater samples and one dog stool, respectively. To our knowledge, this is the first description of the presence of Raccoon CPV-like in water and HAdV-E in lettuce in Southern Brazil.