Abstract
Heterologous radioimmunoassays (RIAs) for the mammalian prodynorphin end products, α-neo-endorphin, dynorphin A(1–17), dynorphin A(1–8), and dynorphin B(1–13) were used to screen brain extracts obtained from representatives of the major surviving orders of reptiles: Chelonia ( Pseudemys scripta), Squamata ( Anolis carolinensis), and Crocodylia ( Alligator mississippiensis). Methanol/acid extracts of whole brains obtained from each species were separately fractionated by gel filtration chromatography and reversed-phase HPLC. In all three species, an immunoreactive form of α-neo-endorphin was detected with the same retention time as synthetic mammalian α-neo-endorphin following reversed-phase HPLC analysis. In all three species, reversed-phase HPLC analysis revealed a novel form of dynorphin B(1–13)-related immunoreactivity. With the available immunological probes, dynorphin A products were only detected in the Anolis brain extracts. Both dynorphin A(1–17) and dynorphin A(1–8) were detected in this species.
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