Abstract
Clavibacter michiganensis subsp. sepedonicus (CMS) is an important bacterial plant pathogen causing potato ring rot disease. Rapid diagnosis of CMS is crucial because of the economic losses caused by serious harvest losses. Although there are serological tests used in the rapid diagnosis of CMS, they are not widely used because of their low sensitivity. The DNA-based PCR methods, which are highly sensitive, do not have the possibility of on-site diagnosis, especially since they require serious laboratory infrastructure. In recent years, scientists have been working on alternative amplification methods to develop DNA-based point of care (POC) diagnostic methods. Accordingly, the loop-mediated isothermal amplification (LAMP) method, which was developed in the early 2000s, provides an important convenience for DNA-based tests to use in the field. Due to the unique design of primers, more amplification products could be create in a shorter time than conventional amplification methods without needing a temperature cycle, and it can be applied with the aid of a simple heater without requiring a laboratory environment. In this study, efficient LAMP method for the detection of CMS has optimized. For device-independent detection of LAMP products, colorimetric method and LFD has used.
Highlights
Clavibacter michiganensis subsp. sepedonicus (CMS) is an important bacterial plant pathogen causing potato ring rot disease
There is a need for a DNA based molecular diagnostic method for the diagnosis of CMS that is suitable for point of care (POC) tests making it viable for use in the field and customs area
It was determined that primers given by the V5 program did not have any specificity against any subtypes of Clavibacter michiganensis
Summary
Clavibacter michiganensis subsp. sepedonicus (CMS) is an important bacterial plant pathogen causing potato ring rot disease. There is a need for a DNA based molecular diagnostic method for the diagnosis of CMS that is suitable for point of care (POC) tests making it viable for use in the field and customs area. At this point, DNA amplification methods that are required for laboratory infrastructure are not suitable for POC tests[18,19]. Free inner primers are bound to this loop region and the reaction becomes continuous[25,26] Because it uses 4 or 6 different primers that recognize 6 or 8 different regions on the target gene, the LAMP method is more sensitive than other PCR methods[27,28,29]. All these detection methods facilitate the use of the LAMP method with point-of-care tests and microfluidic chips[35]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
