Abstract
The surveys conducted during 2006 and 2007 revealed the infection of the virus in potato fields in Tehran province. Due to the important roll of aphids in transmission of the virus, immunocapture reverse transcription polymerase chain reaction (IC-RT-PCR) and reverse transcription polymerase chain reaction (RT-PCR) was developed using potato leafroll virus (PLRV) specific antibodies and specific primer pair (20 mer) located in the virus capsid gene to detect the virus in aphids. A 336-bp PCR product was detected from aphids (Myzus persicae) which had been fed on PLRV-infected plants. The PCR band was specific to PLRV as determined in PLRV-infected plants. This inquiry shows that this method is applicable to assess viroliferous nature of aphids in yellow -pan traps during season.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
