Detection of polypeptides associated with the histopathological differentiation of primary lung carcinoma.

T Hirano,F Vanky,G Auer,L Rodrigues,Aa Alaiya,K Okuzawa,B Franzén,K Uryu,Y Ebihara,H Kato

doi:10.1038/bjc.1995.422

T Hirano, F Vanky + Show 8 more

Open Access

https://doi.org/10.1038/bjc.1995.422

Copy DOI

Abstract

Two-dimensional polyacrylamide gel electrophoresis combined with a non-enzymatic sample preparation technique is useful for analysing clinical tumour material. Using these techniques, we analysed the relationship between the histopathological findings in primary lung malignancies and the expression of a number of unidentified polypeptides that were detected in the molecular weight region 20-35 kDa. In this study 45 cases of primary lung cancer (PLC) (21 cases of adenocarcinoma, ten cases of squamous cell carcinoma, five cases of large-cell carcinoma, one case of adenosquamous cell carcinoma, five cases of small-cell carcinoma and three cases of carcinoid tumour) were examined. For reference, a human diploid fibroblast cell line (W138) and normal peripheral lymphocytes were used. Sixteen polypeptides were judged to be associated with histopathological features. These polypeptides seem to be valuable as differentiation markers. The simultaneous evaluation of these polypeptides and some other proliferation markers (e.g. PCNA, PCNA 'satellite', Numatin/protein B23 and lamin B) seems to clarify the characteristics of each case of PLC. Furthermore, it is possible to classify PLC based on the two-dimensional electrophoresis findings, and this classification of PLC is suggested to reflect the biological features of the tumour more precisely than that based only on morphology.

Highlights

The range of histological appearances of primary lung cancer is extremely wide, even most of such tumours originate in bronchial epithelium
We recently reported that two-dimensional polyacrylamide gel electrophoresis (2-DE) (O'Farrell, 1975) combined with a non-enzymatic sample preparation technique is useful for analysing clinical tumour material (Okuzawa et al, 1994; Franzen et al, 1993)
In three cases of adenocarcinoma (L206, L223 and LTI0) the degree of differentiation was not determined because the histopathological specimen was too small to reflect the characteristics of the entire tumour

Summary

Introduction

The range of histological appearances of primary lung cancer is extremely wide, even most of such tumours originate in bronchial epithelium. Lung cancer is usually classified into small-cell lung cancer (SCLC) and non-small-cell lung cancer (NSCLC). Small-cell lung cancers which contain cytoplasmic dense-core granules resembling neuroendocrine granules, seem to originate from Kultschitzk:y cells. SCLC is characteristic of both neuroendocrine cells and epithelial cells, and the biological behaviour of SCLC is different from that of NSCLC

Objectives

Methods

Results

Discussion

Conclusion