Abstract
Rapid dissemination of plasmid-mediated quinolone resistance (PMQR) has been reported in clinical isolates. A total of 149 clinical isolates of Enterobacteriaceae were collected in Beijing and screened for PMQR genes using polymerase chain reaction (PCR). Real-time quantitative PCR was used to study the expression of qnrS. The rates of qnr and aac(6')-Ib-cr genes were 7.4% and 8.1%, respectively. The higher basal expression of qnrS was observed in transconjugant strains, which had higher minimum inhibitory concentrations (MICs) of quinolones. Furthermore, qnrS expression levels increased in all three isolates when a quinolone was present. Our data suggest that the level of qnrS expression was associated with quinolone resistance.
Highlights
Rapid dissemination of plasmid-mediated quinolone resistance (PMQR) has been reported in clinical isolates
We investigated the presence of PMQR genes in clinical isolates of Enterobacteriaceae, identified mutations within the quinoloneresistance-determining regions (QRDRs), and examined the expression of qnrS in transconjugants obtained from different donor isolates
PMQR genes usingpolymerase chain reaction (PCR) experiments confirmed that the transconjugants harbored the same PMQR determinants as their donors, and that the qnr and aac(6’)-Ib-cr genes could be cotransferred from
Summary
Rapid dissemination of plasmid-mediated quinolone resistance (PMQR) has been reported in clinical isolates. QnrS expression levels increased in all three isolates when a quinolone was present. Conclusions: Our data suggest that the level of qnrS expression was associated with quinolone resistance. Plasmid-mediated quinolone resistance (PMQR) has been reported since 1998 [5]. PMQR determinants confer low-level resistance to quinolones, they are a favorable background for selection of additional chromosome-encoded quinolone resistance mechanisms, which makes the clinical therapy more difficult. We investigated the presence of PMQR genes in clinical isolates of Enterobacteriaceae, identified mutations within the QRDRs, and examined the expression of qnrS in transconjugants obtained from different donor isolates
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